Shimadzu ASMS 2024 Breakfast Seminars
Thank you for your interest in Shimadzu's seminars. Please review information below.
You may register for one or multiple days. We look forward to seeing you in Anaheim.
Location: Anaheim Convention Center
Room 204A (Monday, Tuesday, Wednesday)
Room 204B (Thursday)
Registration has closed. If interested in attending, you may still come that morning and be added to a wait list.
Monday, June 3, 2024
Tackling Metabolic Disease Research at the University of Texas Southwestern Medical Center: The Indispensable Nature of Mass Spectrometry Technologies
Ruth Gordillo, Ph.D., Associate Professor, Director Metabolic Phenotyping Core, UT Southwestern Medical Center, Internal Medicine, Touchstone Diabetes Center
The Metabolic Phenotyping Core (MPC) at UT Southwestern Medical Center offers state-of-the-art analytical and phenotyping services to the broader scientific community. Our mission is to enhance the variety of techniques available to researchers, standardize essential methodologies, and speed up research on diseases associated with metabolic disorders—such as diabetes and obesity—as well as cancer, aging, and neurological disorders. We allocate significant resources to metabolomics research, utilizing our comprehensive mass spectrometry facilities. In this seminar, we will present our most recent metabolomics discoveries, employing advanced LC/MS/MS and MSI technologies to phenotype our mouse models of disease. These efforts aim to shed light on potential disease mechanisms and pathways.
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Tuesday, June 4, 2024
Ultrasensitive Detection and Quantification of Oxygenated Compounds in Complex Samples using GC-combustion-MS
Pierre Giusti - Analytical Chemistry Specialist, TotalEnergies
Oxygen is one of the most abundant components of organic compounds, and its analysis is now essential across a broad range of scientific fields (petroleomics, metabolomics, environmental sciences) and numerous industrial applications (hydrocarbon processing, new energies, natural gas, and biogas, pharmaceuticals, chemicals and additives manufacturing). For this reason, is crucial the detection and quantification of individual oxygen compounds in real complex samples. Current alternatives for oxygen detection, including Oxygen-flame ionization detector (O-FID) or Mass Spectrometry, do not meet the sensitivity, selectivity, and robustness (strongly affected by matrix and quenching effects) requirements needed to detect traces of oxygenates in the complex sample mentioned before. besides, they typically require for specific compound standards for accurate quantification.
Wednesday, June 5, 2024
Next Generation Sample Preparation for MS Imaging: From Saving Resources to Overcoming Research Biases
Dr. Martina Marchetti-Deschmann, Full Professor and Head of the Institute of Chemical Technologies and Analytics at TU Wien, Getreidemarkt 9/164, Vienna, Austria
Fast, cost effective and reproducible workflows for MALDI-MSI experiments is one of the key challenges for the technology to increase market acceptance.
We present our workflow for high spatial resolution MALDI imaging under inert conditions. Matrix preparation protocol are carefully optimised and evaluated for the iMLayer AERO matrix sprayer using the MALDI-8030 benchtop instrument. The linear MALDI instrument is operated with fixed settings and its speed of analysis allows for efficient survey experiments. Matrix selection and deposition parameters can be quickly evaluated in respect to crystal size and spectral quality. The best performing method is then used to prepare samples for high-performance instruments which provide higher spatial and mass resolution (e.g. MALDI-7090). The workflow is proving to be a very efficient tool for developing spraying method or matrix deposition tailored to a specific sample in a very short time.
A key advantage of the iMLayer Aero over other devices is the inert atmosphere during matrix application. We show that sample preparation artefacts such as oxidation can be significantly reduced, minimizing misleading results for biological studies. Oxidation also plays a crucial role in peptide analysis. An example is given an on-tissue digestion study for parchment, where we investigated the impact of environmental pollutants like UV light and SO2 on the alteration of collagen fibers.
Thursday, June 6, 2024
Coupled Supercritical Fluid Extraction and Chromatography with Tandem Mass Spectrometry in the Food Analysis Laboratory: Theory and Applications
Dan Hengst, Principal Scientist, Eurofins Food Chemistry Testing, Madison, WI
Chemists performing nutritional and contaminant analysis of foods, feeds and supplements face a difficult task. They must be prepared with fit-for-purpose methodology suitable for an almost limitless array of matrixes and analytes, with targeted concentrations ranging from part per trillion type levels to 100% analyte composition. Many of the targeted nutritional and contaminant compounds will have unique challenges, such as instability and the presence of isomers and isobaric interferences. Since product release to the market may be based upon analytical results meeting a specification range, the analytical food chemist works in a stressful, high-paced laboratory environment with tight deadlines. These challenges have led to the development of rugged, high-throughput instruments with methodology suitable for a wide array of sample types. An example of such a technique is Coupled Supercritical Fluid Extraction and Chromatography with Tandem Mass Spectrometry Detection, which utilizes supercritical Carbon Dioxide to simultaneously extract, resolve, detect and quantitate targeted analytes from sample matrix. Numerous advantages are seen with this platform, including reduced laboratory labor, rapid sample throughput, preservation of instable analytes, low cost of operation and reduced usage of hazardous solvents. This presentation focuses on such an instrument: the Shimadzu Nexera SFE-SFC/8060NX MSMS as utilized for the analysis of the Fat-Soluble Vitamins.